A major technical challenge in developing Anti-Drug Antibody (ADA) assays is to overcome drug tolerance (DT). High levels of therapeutic drugs in matrix sample can mask ADAs, making them undetectable. This interference occurs because the drug can bind to the antibodies, forming complexes that the assay cannot detect. To overcome DT in ADA assays, sample pretreatment is needed to clear drug from the sample. Examples of pretreatment options include acid dissociation, Solid Phase Extraction and Acid Dissociation (SPEAD), Affinity Capture Elution (ACE), or Precipitation and Acid Dissociation (PandA).
Learning Objectives:
Upon completion, participant will be able to understand the use of sample pretreatment in ADA assays to overcome drug tolerance.
