Description: A direct analysis of drug substances is often not feasible due to lack of detection techniques. Derivatization coupled with chromatographic instrumentation, primarily HPLC and GC, allows to increase sensitivity of the method by employing typical ultraviolet and flame ionization detectors correspondingly. Different derivatization reagents are available for pre-column alkylation and acylation of different classes of compounds, including amines, carbamates, aldehydes, and hydroxy compounds.
Learning Objectives:
Review common derivatization techniques including those for special applications such as analysis of chiral purity and determination of impurities at ppm level.
During this session, participants will discuss case studies focusing on real examples in pharmaceutical analysis.
Upon completion, participants will be able to apply known and novel derivatization procedures for analysis of multiple drug substances.
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